Exploring the therapeutic potential of TGF-β in albino mice infected with hydatid cyst using immunohistofluorescence analysis

Abstract

This study was designed to rate the efficacy of different antiparasitics against secondary echinococcosis in albino Mus musculus Balb/c borrowed laboratory IHF (immunohistofluorescence) staining method. Mice were experimentally infected with hydatid cysts and then treated with oxfendazole (OFZ), praziquantel (PZQ), and albendazole (ABZ) at 30 mg/kg, 40 mg/kg, and 10 mg/kg body weight, respectively. Treatment was administered once weekly for four months. The combination of OFZ + PZQ treatment presented the highest level of therapeutic efficiency; the other treatments presented descending levels in the sequence: OFZ only, OFZ + ABZ, and ABZ + PZQ. The IHF staining aimed at detection and evaluation of expression for TGF-β3 cytokine within liver and spleen tissues obtained from these animals. Oxfendazole can be considered an encouraging drug under the findings observed for hydatid disease.

Keywords:

TGF-β3, Echinococcus granulosus, Oxfendazole, hydatid cysts

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