The Significance of Serum IL-10 Levels in Diagnosing Hepatitis B Virus (HBV) Infection

Abstract

Background: Cytokine secretion is one of the most immunological affecters that play a major role in the pathogenesis hepatitis B and, a member of these anti-inflammatory cytokines with unique characteristic is interleukin-10 (IL-10). Objective: To evaluate the levels of IL-10 in clients with hepatitis B infections is the primary objective of the present study. Methods: A case–control study design was developed consisting of 62 laboratory-confirmed hepatitis B infection subjects and 58 controls who were apparently healthy. Enzyme-linked immunosorbent assay (ELISA) was performed to assess serum IL-10 levels. Differences between groups were investigated via independent samples t-tests; differences by infection site were assessed with analysis of variance (ANOVA). Furthermore, the diagnostic value of IL-10 was evaluated using receiver operating characteristic (ROC) curve analysis for the diagnosis of infected versus non-infected. Results: The IL-10 was significantly increased in patients than controls [18.8 ± 32 vs. 15.9 ± 3.9 PG/ml (p <30]. Elevation of serum IL-10 levels in relation with vaccination status suggested higher concentrations in non-vaccinated patients with hepatitis B infections (19.2 ± 3.2 pg/ml) than those vaccinated (13.5 ± 2.8 pg/ml). The AUC was 0.78, established an optimal diagnostic threshold at 16 pg/ml (sensitivity: 82%, specificity: 73%). Further, IL-10 was identified as a potential biomarker for hepatitis B by showing the average serum level of it increased significantly in patients with hepatitis B.

Keywords:

Interleukin-10, hepatitis B, AUC, Sensitivity, Specificity

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