Effects of Alcoholic Extract Nigella Sativa on Blood Clotting Markers in Male Rats Models with F2 G20210A Mutation

Abstract

Despite its well-documented antioxidant and anti-inflammatory activities, there are no sufficient data exists regarding duration of Nigella sativa effects on blood coagulation. This experiment observed the effects of a sixty-day oral intoxication of alcoholic Nigella sativa extract on four key hemostatic indicators—prothrombin time, fibrinogen, D-dimer and platelet count—with respect to the veins of normal male rats treated or otherwise and rats carrying the F2 G20210A mutation as a model of increased thrombotic risk. Thirty mature male rats were divided into three groups: control, normal rats treated with N. sativa extract, and rats crossing the G20210A mutation into their genome and therefore in theory at higher risk of thrombosis. After 60 days of treatment, blood was taken for coagulant analysis. The treated normotensive rats displayed a significant prolongation of PT and reductions in fibrinogen and D-dimer levels, pointing to an anticoagulant action of the extract. However, the group disadvantaged by the mutation presented with smaller PT, greater fibrinogen, higher D-dimer and peaked platelet levels, thus manifesting a hypercoagulative profile. The results generally indicate that long-term exposure to alcoholic Nigella sativa may to some extent influence coagulation in such a way as to lessen the tendency toward clot formation and that when there is a prothrombin gene mutation present, a strong prothrombotic condition still prevails. Altogether, these results emphasize the potential of Nigella sativa to act as a natural modulator of coagulation, particularly in the absence of genetically derived hypercoagulable circumstances.

Keywords:

Nigella sativa, Prothrombin Time, Fibrinogen, D-Dimer, Platelet Count

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