Laboratory Investigations in Patients with Hypothyroidism and Compared with Healthy Control Group in Baquba Teaching Hospital

Abstract

Background: One of the utmost prevalent endocrine disorders is hypothyroidism, which can reason moderate symptoms like depression, cold sensitivity, fatigue, and weight gain, as well as further severe one’s alike myxedema and mortality.

Objective: To assess the laboratory investigations in patients with hypothyroidism and compared with healthy control group in Baquba Teaching Hospital.

Method: Cross sectional study was fixed amongst 90 individuals (60 patients with hypothyroidism and 30 healthy control) from Baquba Teaching Hospital, Diyala. The study was finished in the dated from December 2024 to February 2025. We estimated thyroid function (T3, T4, TSH) and hematological analysis (WBC, RBC, and platelets count) to find the relationship between them and compared with healthy control.

Results: The results exhibited count of WBC in patients’ group was statistically non-significant lessened as associated with control group. The RBC count displayed enlarged level in control group as associated to patients with statistically exposed no significant connection. The platelets count presented enlarged level in control group as related to patients with statistically exhibited no significant connection. The results illustration Mean ±SE of platelets counts in patient’s male more than in female with statistically significant relationship. The patients were separated into three age groups, (<40 years), (40-50 years) and (>50 years) and all hematological analysis were divided into the age groups. It was shown through the statistical results that age does not affect the results of the hematological test.

Conclusion: The study found that thyroid issues affect women more often than they do men. It is crucial for hematological testing on hypothyroid patients.

Keywords:

Patients, hypothyroidism, hematological, analysis.

References

Y. Song, C. Massart, V. Chico-Galdo, L. Jin, V. De maertelaer, C. Decoster, J. E. Dumont, and J. Van Sande, “Species specific thyroid signal transduction: conserved physiology, divergent mechanisms,” Molecular and Cellular Endocrinology, vol. 319, pp. 56–62, 2010. https://doi.org/10.1016/j.mce.2010.01.024

yen PM. Physiological and molecular basis of thyroid hormone action. Physiol Rev. 2001;81(3):1097–142. https://doi.org/10.1152/physrev.2001. 81.3.1097

Taylor PN, Albrecht D, Scholz A, Gutierrez Buey G, Lazarus JH, Dayan CM, et al. Global epidemiology of hyperthyroidism and hypothyroidism. Nat Rev Endocrinol. 2018;14(5):301–16. https://doi.org/10.1038/nrendo.2018. 18

Unnikrishnan AG, Kalra S, Sahay RK, Bantwal G, John M, Tewari N. Prevalence of hypothyroidism in adults: An epidemiological study in eight cities of India. Indian J Endocr Metab. 2013;17:647–52. 3. doi: 10.4103/2230-8210.113755

Unnikrishnan AG, Menon UV. Thyroid disorders in India: An epidemiological perspective. Indian J Endocr Metab. 2011;15(Suppl S2):78–81. doi: 10.4103/2230-8210.83329

Idan, H. M., & Tofiq, S. H. (2024). Oral manifestations and hemoglobin level in children with subclinical hypothyroidism. Journal of Emergency Medicine, Trauma & Acute Care, 2024(8), 13.‏ https://doi.org/10.5339/jemtac.2024.midc.13

Brent GA. Clinical practice. Graves’ disease. New Engl J Med. 2008;358(24):2594–2605. doi: 10.1056/NEJMcp0801880

Pillai, N. S., & Bennett, J. (2018). Prevalence of hypothyroidism amongst pregnant women: a study done in rural set up. International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 7(4), 1586-1592.‏ DOI: https://doi.org/10.18203/2320-1770.ijrcog20181360

Saikh MG, Anderson JM. Transient neonatal hypothyroidism due to maternal vegan diet. J Pediatr Endocrinol Metab. 2003;16:111-3. doi:10.1515/jpem.2003.16.1.111

SPSS (2019). Statistical Packages of Social Sciences-SPSS/ IBM Statistics 26 step by step. 16th Edition. https://doi.org/10.4324/9780429056765.

T. H. Fitzpatrick and M. A. Siccardi, Anatomy, Head and Neck, Adam’s Apple. 2018.

S. Corbetta, “Classification of thyroid diseases,” Thyroid, Obesity and Metabolism: Exploring Links Between Thyroid Function, Obesity, Metabolism and Lifestyle, pp. 21–35, 2021.

Ahmed, S. S., & Mohammed, A. A. (2020). Effects of thyroid dysfunction on hematological parameters: Case controlled study. Annals of Medicine and Surgery, 57, 52-55.‏ https://doi.org/10.1016/j.amsu.2020.07.008

Dorgalaleh, M. Mahmoodi, B. Varmaghani, Effect of thyroid dysfunctions on blood cell count and red blood cell indice, Iranian J. Pediatric Hematol. Oncol. 3 (2) (2013) 73.

M. Iddah, et al., Thryroid Hormones and Hematological Indices Levels in Thyroid Disorders Patients at Moi Teaching and Referral Hospital, Western Kenya vol. 2013, ISRN endocrinology, 2013. https://doi.org/10.1155/2013/385940

P.K. Kamdar, A.V. Mendpara, TO study hematological abnormalities IN patients OF thyroid dysfunction, Int. J. Sci. Res. 8 (12) (2020).

Kadgi, N. V., Chauhan, S. G., & Nakate, L. A. (2021). Hematological changes in hypothyroidism and hyperthyroidism in adults. Indian J Pathol Oncol, 8(4), 452-456.‏ https://doi.org/10.18231/j.ijpo.2021.094

A.A. Erikci, et al., The effect of subclinical hypothyroidism on platelet parameters, Hematology 14 (2) (2009) 115–117. https://doi.org/10.1179/102453309X385124

R.S. Chandel, G. Chatterjee, L.G. Abichandani, Impact of subclinical hypothyr oidism on iron status and hematological parameters, Ann Pathol Lab Med 2 (2015) A21–A25.

Jafarzadeh, et al., Immunological and hematological changes in patients with hyperthyroidism or hypothyroidism, Clin. Investig. Med. (2010) E271–E279. https://doi.org/10.25011/cim.v33i5.14352

Lima CSP, Wittmann DEZ, Castro V, Tambascia MA, Lorand-Metze I, Saad STO, et al. Pancytopenia in untreated patients with Graves’ disease. Thyroid. 2006;16(4):403–9. https://doi.org/10.1089/thy.2006.16.403

Kawa MP, Grymuła K, Paczkowska E, Bakiewicz-Masiuk M, D˛ abkowska E, Koziołek M. Clinical relevance of thyroid dysfunction in human haematopoiesis: biochemical and molecular studies. Eur J Endocrinol. 2010;162(2):295–305. https://doi.org/10.1530/EJE-09-0875

T.S. Ashraf, et al., Chronic anemia and thyroid function, Acta Biomed.: Atenei Parmensis 88 (1) (2017) 119. doi: 10.23750/abm.v88i1.6048

R.K. Schindhelm, et al., Thyroid hormones and erythrocyte indices in a cohort of euthyroid older subjects, Eur. J. Intern. Med. 24 (3) (2013) 241–244. https://doi.org/10.1016/j.ejim.2012.12.004

Idan, H. M., Hasani, R. A. M., & Othman, I. Q. (2024). Effect of anemia on oral cavity and hematological assessments. Diyala Journal of Medicine, 26(1), 153-162. ‏https://doi.org/10.26505/djm.v26i1.1087